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RPE65 – retinal degeneration: for patients

Overview

Mutations in the RPE65 gene lead to progressive degeneration of the retinal photoreceptor (light-sensing) cells in children. It is one of the major causes of Leber congenital amaurosis and a small proportion of retinitis pigmentosa cases.[1] Night blindness or difficulty adapting to dark conditions from early childhood is a prominent symptom. Other features include involuntary movement of the eyes (known as nystagmus), reduced central visual sharpness (visual acuity) and loss of peripheral vision of variable severity. Despite difficulties seeing in the dark, children may still retain some vision in bright environments up to early teenage years before progressive deterioration thereafter but this can vary among patients within and between families. As of 2019, UK patients with retinal degeneration due to mutations in RPE65 can be treated with a form of gene therapy called Luxturna under the National Health Service (NHS).

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The condition

Symptoms

The condition usually starts during early childhood, sometimes even as young as within the first few months of life, where parents may notice poor visual behaviour in their child which is usually accompanied by nystagmus. Night blindness is a prominent symptom in which the child may seem afraid of being in the dark or dim environment but tends to behave normally during daytime or in a brightly-lit room. Furthermore, there is normally a reduction in visual sharpness and loss of peripheral vision but some may have sufficient vision to function normally in a well-lit environment during their infant and primary school years.[2-5]

Vision tends to remain stable up to early teenage years before deteriorating progressively thereafter, leading to “tunnel vision” which is eventually lost by middle age.[3] The severity of these symptoms and the rate of visual decline are highly variable, but children with earlier onset of visual symptoms tend to be more severely affected.[6] Rarely, some patients may only start experiencing symptoms from adolescence onwards in which case visual function might not deteriorate as rapidly as usual.[7-9]

A person driving a car with normal peripheral vision is able to see the girl in a red dress on the left. If the driver has a loss of peripheral vision, the same girl is not noticeable.
Blind spot in the peripheral vision
Seeing from the perspective of a patient with tunnel vision. The side vision is blurry while in the centre a clear view of a town with building can be seen.
Tunnel vision

Cause

The RPE65 gene instructs cells to produce a protein known as retinoid isomerase. It helps the photoreceptor cells in the retina to convert incoming light into electrical signals that are transmitted to the brain to generate sight through a series of chemical process called the visual cycle. When the RPE65 gene is not working properly, this process cannot be undertaken which leads to sight loss.

A microscopic view of the cells in the retina. The cone-shaped and rod-shaped photoreceptors are situated deepest and supported by the RPE cells. Other cells above the photoreceptors are responsible for transmitting electrical signals to the brain to generate vision.
Microscopic view of the cells in the retina: The rod and cone photoreceptors are at the bottom supported by the retinal pigment epithelium. The other cell types above the photoreceptors relay electrical signals to the brain

How is it diagnosed?

The condition is diagnosed based on the symptoms, family history and clinical evaluation of the retina. As it can appear similar to inherited retinal degenerations caused by other genes, the diagnosis can only be confirmed with genetic testing by identifying mutations in the RPE65 gene.

How is it inherited?

1) Autosomal recessive (AR) inheritance

Most cases of RPE65-related retinal degeneration are inherited in this manner. Both parents are usually unaffected carriers (who only carry one faulty copy of the gene) while the patient has two faulty gene copies (inherited one each from each parent). This means that every newborn has the following risks regardless of gender:

  • 25% chance of being affected by the condition
  • 25% chance of being unaffected and not a carrier
  • 50% chance of being a carrier with no symptoms
If the mother has 1 copy of the faulty gene in her X chromosome (a carrier) while the father is unaffected, there is 50% chance that a daughter is a carrier and a 50% chance that a son is affected by the condition.
Autosomal recessive inheritance

2) Autosomal dominant (AD) inheritance

A very small number of cases are inherited in this manner. Only one copy of the faulty gene (inherited from either parent) is required to cause disease. This means that each newborn of the patient has a 50% chance of inheriting the condition regardless of gender. It usually causes a later-onset disease with less severe visual deterioration than if there are two faulty copies of the RPE65 gene.

The faulty gene copy is present in the father while the mother is not affected. Each newborn of this couple has a 50% chance to be affected by the condition.
Autosomal dominant inheritance

If you or your child is affected by RPE65-related retinal degeneration, it is advisable to see a genetic counsellor to obtain more information and advice on inheritance and family planning options.

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Treatment

Is there any treatment?

1) Gene therapy

Gene therapy aims to halt retinal degeneration by replacing the mutated gene with a normal healthy copy. This enables the affected cells to regain some of their function and produce functioning proteins.

Patients with RPE65-related retinal degeneration are now able to receive a treatment called Luxturna (voretigene neparvovec) under the NHS. In Luxturna, a normal copy of the RPE65 gene is “packaged” into a harmless virus called the adeno associated virus (AAV) which is then surgically injected into the retina (sub-retinal injection). This way, the affected retinal cells are maximally exposed to the viruses containing the normal gene. 

The normal gene copy is packaged into a harmless virus, which is then injected below the retina through surgery. This allows maximum exposure of the photoreceptors to the injected therapy
Sub-retinal injection

This was introduced following the success of a clinical trial where patients treated with Luxturna in both eyes (sequentially at different periods) have improved visual function and navigational abilities at low light levels compared to those who did not receive treatment.[10] This improvement has been sustained up to 4 years after the initial surgery.

In the UK, patients can be referred to the following treatment centres for consideration of Luxturna treatment:

The main goal of Luxturna is to halt progressive retinal degeneration and thus stabilises remaining vision. The positive results reported from the aforementioned trial may not be observed in every patient and some may not be eligible for treatment as there may be insufficient healthy photoreceptor cells remaining or other factors. Therefore, we strongly emphasise that interested patients or parents should have a thorough discussion with an ophthalmologist familiar with this procedure before proceeding.

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2) Supportive treatment

For those who may not want to proceed with gene therapy or not eligible, treatment is focused on limiting additional damage to the retina and optimising remaining sight:

  • Regular eye examinations to correct any long- (hyperopia) or short-sightedness (myopia)
  • Referral to low vision services
  • Visual aids and assistive technology
  • Have a healthy diet consisting of fresh fruits and green leafy vegetables
  • Wear UV protected sunglasses in bright light
  • Using blue light screen protectors on mobile devices or computer screens*

*Current available evidence shows that blue light emitted from screens do not damage the retina but it can disrupt our sleep cycle. The screen protectors are used as a precautionary measure.

3) Optimising development

Visual impairment can have a negative impact on a child’s early general development. Therefore, timely referral to practitioners familiar with developmental surveillance and intervention for children with visual impairment (VI), such as developmental paediatricians as well as a Qualified Teacher of children and young people with Visual Impairment (QTVI) is crucial to optimise their developmental potential.

The Developmental Journal for babies and young children with visual impairment (DJVI), developed by Great Ormond Street Hospital Developmental Vision team is a structured early intervention programme designed to track developmental and vision progress in children from birth to three years of age. It is mainly used by qualified healthcare professionals working in services providing support to babies and young children with VI in conjunction with the child’s parents. Children with VI may be referred to developmental services such as the developmental vision clinic in the Great Ormond Street Hospital for Children or other specialist developmental services for further management.

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Current research in RPE65-retinal degeneration

1) Gene therapy

Although Luxturna is now an approved treatment, a second-generation gene therapy called AAV2/5-OPTIRPE65 is being developed to increase the efficiency of transferring the normal RPE65 gene into the retinal cells. A phase 1/2 trial is being conducted and the interim results show that its safety profile was consistent with gene therapies under research for other eye conditions.

Related links

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Practical advice

Living with RPE65-retinal degeneration

Patients are still able to lead fairly independent lives through maximising their available vision and having access to social support. Here are some ideas:

  • Attending the low vision clinic which provides access to low vision specialists, Eye Clinic Liaison Officers (ECLOs), visual aids and visual rehabilitation services
  • Utilising assistive technologies that can improve quality of life
  • Getting in touch with the local education authority for access to qualified teachers for children with visual impairment (QTVI) and special educational needs co-ordinator (SENCO)
  • Registering your child as sight impaired (SI) or severely sight impaired (SSI) if eligible for access to social support and financial concessions
  • Getting in touch with national or local charities for advice and peer support

Related links

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Referral to a specialist centre

If you are based in the UK and would like to be seen in the nearest specialist centre for your eye condition, either to receive a more comprehensive genetic management or just to find out more about current research, you can approach your GP to make a referral or alternatively arrange for a private appointment. 

More information can be found in our “How to see a genetic eye specialist?” page.

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Further information and support

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A patient’s perspective

A family’s experience with RPE65 associated LCA and how treatment with Luxturna changed their lives

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References

  1.  Morimura H, Fishman GA, Grover SA, Fulton AB, Berson EL, Dryja TP. Mutations in the RPE65 gene in patients with autosomal recessive retinitis pigmentosa or leber congenital amaurosis. Proc Natl Acad Sci U S A. 1998;95(6):3088-3093
  2.  Thompson DA, Gyürüs Pt, Fleischer LL, et al. Genetics and Phenotypes of RPE65 Mutations in Inherited Retinal Degeneration. Investigative Ophthalmology & Visual Science. 2000;41(13):4293-4299
  3.  Chung DC, Bertelsen M, Lorenz B, et al. The Natural History of Inherited Retinal Dystrophy Due to Biallelic Mutations in the RPE65 Gene. Am J Ophthalmol. 2019;199:58-70
  4.  Weleber RG, Michaelides M, Trzupek KM, Stover NB, Stone EM. The phenotype of Severe Early Childhood Onset Retinal Dystrophy (SECORD) from mutation of RPE65 and differentiation from Leber congenital amaurosis. Invest Ophthalmol Vis Sci. 2011;52(1):292-302
  5.  Li S, Xiao X, Yi Z, Sun W, Wang P, Zhang Q. RPE65 mutation frequency and phenotypic variation according to exome sequencing in a tertiary centre for genetic eye diseases in China. Acta Ophthalmol. 2019
  6.  Zahid S, Branham K, Schlegel D, et al. Retinal Dystrophy Gene Atlas. Springer; 2018
  7.  Bowne SJ, Humphries MM, Sullivan LS, et al. A dominant mutation in RPE65 identified by whole-exome sequencing causes retinitis pigmentosa with choroidal involvement. Eur J Hum Genet. 2011;19(10):1074-1081
  8.  Hull S, Holder GE, Robson AG, et al. Preserved visual function in retinal dystrophy due to hypomorphic RPE65 mutations. Br J Ophthalmol. 2016;100(11):1499-1505
  9.  Jauregui R, Park KS, Tsang SH. Two-year progression analysis of RPE65 autosomal dominant retinitis pigmentosa. Ophthalmic Genet. 2018;39(4):544-549
  10.  Russell S, Bennett J, Wellman JA, et al. Efficacy and safety of voretigene neparvovec (AAV2-hRPE65v2) in patients with RPE65-mediated inherited retinal dystrophy: a randomised, controlled, open-label, phase 3 trial. Lancet. 2017;390(10097):849-860

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Updated on December 8, 2020
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